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Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 20 , ECs Cell Culture Medium Complete Phenol free , Cell Biologics Inc , M1168PF , ECs culture medium without phenol red used during LDH measurement.

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 23 , ECs Hypoxia Cell Culture Medium , Cell Biologics Inc , GPF1168 , ECs cell culture under hypoxic condition (glucose- and serum-free).

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

(EC; 25: 25,000 cells per insert; 50: 50,000 cells per insert; 100: 100,000 cells per insert) dose-dependently affected protection of cardiomyocytes (CM) against hypoxia/reoxygenation injury (right) compared to normoxic conditions (left) as evidenced by release of lactate dehydrogenase (LDH; in absorbance units [au]). ECs had no effect on LDH release under normoxic conditions. Data are mean ± standard deviation, n=4 per group. Statistics: ANOVA followed by Student-Newman-Keuls post-hoc test. P < 0.05 (two-tailed) indicated by horizontal bars between each compared pair.

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet: (EC; 25: 25,000 cells per insert; 50: 50,000 cells per insert; 100: 100,000 cells per insert) dose-dependently affected protection of cardiomyocytes (CM) against hypoxia/reoxygenation injury (right) compared to normoxic conditions (left) as evidenced by release of lactate dehydrogenase (LDH; in absorbance units [au]). ECs had no effect on LDH release under normoxic conditions. Data are mean ± standard deviation, n=4 per group. Statistics: ANOVA followed by Student-Newman-Keuls post-hoc test. P < 0.05 (two-tailed) indicated by horizontal bars between each compared pair.

Article Snippet: 23 , ECs Hypoxia Cell Culture Medium , Cell Biologics Inc , GPF1168 , ECs cell culture under hypoxic condition (glucose- and serum-free).

Techniques: Standard Deviation, Two Tailed Test

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 19 , ECs Cell Culture Medium Complete , Cell Biologics Inc , M1168 , ECs culture complete medium.

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline